cviaii restriction enzyme (New England Biolabs)
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cviaii restriction enzyme
Cviaii Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cviaii/pmc10848941-189-11-14
Average 86 stars, based on 1 article reviews
Cviaii Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cviaii/pmc10848941-189-11-14
Average 86 stars, based on 1 article reviews
cviaii restriction enzyme - by Bioz Stars,
2026-10
86/100 stars
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other:Article Title: Global loss of promoter–enhancer connectivity and rebalancing of gene expression during early colorectal cancer carcinogenesis Article Snippet: Organoids were cultured in a complete medium (advanced DMEM/F12 supplemented with 10 mM HEPES, 1× Glutamax, 1% penicillin–streptomycin, 1× B27 without vitamin A, 10 mM nicotinamide, 1.25 mM N -acetylcysteine, 500 nM A83-01 (Tocris), 10 μM SB202190 (Sigma-Aldrich), 100 ng μl −1 Noggin (R&D Systems), 1 μg ml −1 human recombinant R-spondin (Stemcell), 0.3 nM Wnt-FC (Immunoprecise), 50 ng ml −1 EGF (Shenandoah Biotechnology), 2.5 μM CHIR 99021 (Tocris) and 100 μg ml −1 Normocin (InvivoGen). Derivative Assay:Article Title: Ultrafine mapping of chromosome conformation at hundred basepair resolution reveals regulatory genome architecture. Article Snippet: To carry out triple digestion, the suspension was mixed with 50 μL of buffer G (ThermoFisher), 120 U MboI (DpnII) (Thermo Fisher ER0811, 10 U/μL), 120 U Csp6I (CviQI) (Thermo Fisher ER0211, 10 U/μL), 100 U Hin1II (NlaIII) (Thermo Fisher ER1831, 5 U/μL), and x μL of water, where x is determined to bring the total volume to 500 μL, empirically within a range of 100 to 150 μL, depending on the pellet size. .. The genomic triple digestion can be alternatively performed by using a combination of MboI (Thermo Fisher), Csp6I (Thermo Fisher), and Suspension:Article Title: Ultrafine mapping of chromosome conformation at hundred basepair resolution reveals regulatory genome architecture. Article Snippet: The genomic triple digestion can be alternatively performed by using a combination of MboI (Thermo Fisher), Csp6I (Thermo Fisher), and CviAII (New England Biolabs) to generate consistent 5′ TA overhangs for other 3C- derived protocols requiring biotin- dA filling. .. In this case, after Triton quenching, the nuclei suspension was mixed with 50 μL of 10× CutSmart Buffer (NEB) and 100 U Article Title: Global loss of promoter-enhancer connectivity and rebalancing of gene expression during early colorectal cancer carcinogenesis. Article Snippet: After another centrifugation, the purified nuclei were resuspended in 170 μl of 10 mM Tris-HCl containing 0.5% Triton X-100 (Sigma, 93443). .. This was followed by incubation at room temperature with rotation for 15 min. Then, 10 μl of 1% SDS, 20 μl of Cutsmart buffer (New England Biolabs (NEB)), 3 μl each of HinP1I (NEB, R0124S), DdeI (NEB, R0175L), Incubation:Article Title: Global loss of promoter-enhancer connectivity and rebalancing of gene expression during early colorectal cancer carcinogenesis. Article Snippet: After another centrifugation, the purified nuclei were resuspended in 170 μl of 10 mM Tris-HCl containing 0.5% Triton X-100 (Sigma, 93443). .. This was followed by incubation at room temperature with rotation for 15 min. Then, 10 μl of 1% SDS, 20 μl of Cutsmart buffer (New England Biolabs (NEB)), 3 μl each of HinP1I (NEB, R0124S), DdeI (NEB, R0175L), |
